Rapid development methods of preparative large thin-layer chromatography (TLC).
نویسندگان
چکیده
منابع مشابه
Preparative Thin-Layer (Planar) Chromatography
The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...
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n Thin-layer chromatography (TLC) is being used increasingly for the separation of the lipid classes (1, 2) prior to the analysis of their fatty acids by gas-liquid chromatography (GLC). Malins and Mangold (3) have described the separation of the methyl esters of fatty acids of menhaden oil from other lipid constituents and stated “the gas chromatogram of the total methyl ester fraction agreed ...
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We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The ad...
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Biological membranes separate cells from the environment. From a single cell to multicellular plants and animals, glycerolipids, such as phosphatidylcholine or phosphatidylethanolamine, form bilayer membranes which act as both boundaries and interfaces for chemical exchange between cells and their surroundings. Unlike animals, plant cells have a special organelle for photosynthesis, the chlorop...
متن کاملLarge-Scale, Preparative Paper Chromatography
Most of the techniques that have been described for large-scale, preparative paper c hromatograp hy depe nd on an i.ncrease in bulk through the use of many closely pressed layers of a thin paper [IJ.' So me of these methods require the tedious loading of each s hee t , and others tend to suffer from a lack of uniformity in the movement of compounds thro ughout the bulk. Th e latter disadvantage...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1990
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.28.59